PSXIII-B-18 Temporal Expression of Interleukin 6: Myokine Protein Abundance in Primary Bovine Satellite Cells from 11-Month-old Beef Steers

Abstract Myokine abundance and function have not been thoroughly evaluated in beef cattle. Many myokines shown to a substantial impact on the health well-being of mammals. While interleukin 6 (IL-6) is known immune cytokine, it also classified as myokine mice produced from muscle contraction post-exercise. IL-6 linked hypertrophy through action increasing glucose uptake stimulating satellite cell proliferation. The objective this study characterize temporal production by cultured primary bovine cells (BSCs) 11-month-old steers. was quantified vitro undifferentiated BSCs isolated This experiment had 3 experimental replicates where were plated triplicate with 10% Fetal Bovine Serum DMEM. At 70% confluency growth media changed, time zero. then remained unchanged collected temporally at 0, 12, 24, 48 hours. Media assayed using ELISA’s quantify abundance. Cells harvested for mRNA extraction analyses future time. We are initial stages analyses, though preliminary data indicate no difference between BSC pools different Temporally, we report that hour 0 there undetectable concentration, but hours steady concentration released into BSCs. confirms being post-weaned, conclude myokine; involved post-natal skeletal